Dr. Will Lai gave a talk on Methods of defining “success” in ChIP-seq/exo experiments on Saturday December 9, 2018 at the The Conference on Regulatory and Systems Genomics with DREAM Challenges 2018. The conference was held at NYU Langone Health, in New York City, New York.
Dr. Pugh has now been appointed as the Director of the EGR Training Grant!
This NIH-sponsored Training Program prepares young scientists to excel in both experimental and computational approaches to address fundamental questions in gene regulation. Nearly all aspects of biology and human disease are rooted in gene regulation. Students in the program are vital members of the Center for Eukaryotic Gene Regulation (CEGR) community, with critical expertise in biophysics, biochemistry, molecular biology, genetics, computational biology, and statistics. Trainees are supported for a period of two years as a part of their doctoral programs.
Please click on the link to read the news story about this training grant!
October 4th – 8th, 2018- Dr. Pugh attended the American Society for Biochemistry and Molecular Biology Conference in Snowbird, Utah to discuss Transcriptional Regulation by Chromatin and RNA Polymerase II.
October 3rd and 4th, 2018- Dr. Pugh traveled to Madison, Wisconsin for the 3rd Annual Wisconsin Epigenetics Group Symposium to discuss the lab’s the research on epigenetics and gene regulation.
July 22 – 26, 2018- Dr. Pugh traveled to Newry, ME to attend the Gordon Research Conference on Chromatin Structure and Function. The title of Dr. Pugh’s talk was Genomic Mechanisms of Gene Regulation and Chromatin Organization.
The 2018 Gordon Conference on Chromatin Structure and Function will bring together leading experts and newcomers to the field, to present and discuss their newest research on chromatin, its complex interactions, and its regulatory power in shaping genome architecture and function. Technologies to visualize and map chromatin at all levels are proceeding at a rapid pace. The meeting will highlight how chromatin structures, ranging from the nucleosome – the “building block” of chromatin – up to the dynamic three-dimensional spatial organization of the chromosome, are assembled, maintained, and altered, and how this ultimately controls genome functions.
Interconnected questions at the frontier of the field will be addressed – how does the nucleosome interact with other nuclear factors, how is chromatin assembled, disassembled, and maintained in a dynamic state, what is the molecular basis for the crosstalk between epigenetic modifications and metabolism, and how does dysfunction of any of these processes result in disease.
Presentations will encompass a wide variety of innovative technical approaches – from Angstrom level description of chromatin components at all organizational levels to the visualization of micron-scale structures by light microscopy, and the mapping of chromatin states genome-wide at unprecedented resolution. There will be a focus on new methods to determine chromatin dynamics and the relationships between chromatin structure and function, and on adapting these to the level of single molecules and single cells.
This conference has a long history of attracting the top researchers in chromatin research, and has a history of bringing in “players” from related fields. It always has been an important forum for fostering new ideas, interactions and collaborations, and this tradition will be continued in 2018. Conference attendees traditionally stay for the duration of the meeting, and the presentation of unpublished material is strongly encouraged. We will continue to integrate new members into this growing field; a large number of talks will be reserved for young scientists and for under-represented minorities, and all applicants will have the opportunity to present a poster on their work. Many platform presentation topics will be selected from submitted abstracts to ensure inclusion of the latest developments during the formal sessions. Additionally, “Poster Previews” will highlight selected posters in short oral presentations. Sufficient discussion time will be scheduled into every session to promote the lively and free exchange of ideas and interpretations and there will be ample time set aside in the afternoons and after the evening sessions for the informal interactions that have always played a key role at this conference. As a new feature this year, the GRC will be preceded by a GRS which we expect to further enhance interactions and networking among the younger conference participants.
Here at Pugh lab we care about building good community. That is why each year we plan various group activities outside of the lab, such as our annual lab outing. This year for our annual lab outing we camped at Ricketts Glenn, followed by an early Fourth of July barbecue. Please enjoy some picture from our outdoor adventure! Central Pennsylvania is a beautiful place to live and work!
We are looking for talented and motivated individuals who want to be a part of the epigenomics revolution!
We developed and use the ultra-high resolution ChIP-exo mapping technique.1 Most notably, we are using yeast and human systems to define the positional and mechanistic organization of all proteins that interact with the genome. Knowledge of protein positional organization informs of biological mechanisms that we can test using various gene editing methods.2 In human systems we look at many cell types, including clinical samples that are prepared and sequenced here in Dr. Pugh’s lab. Dr. Mahony’s lab is also investigating neuronal cells.3 What is most exciting is integrating thousands of datasets into a coherent view of genome regulation. This is what is most challenging also, as it involves developing advances in computational and statistical methods. Read more Recruiting new talent to PughLab!
How to apply for a Postdoc Position:
- Please send a Cover Letter, CV, and Letters of Recommendation to our Principal Investigator, Frank Pugh, at firstname.lastname@example.org
How to apply to a Graduate Program:
- Biochemistry and Molecular Biology Application Page
- Molecular Cellular and Integrative Bio-Sciences Application Page
- Bioinformatics and Genomics Application Page
The Penn State’s Undergraduate Exhibition communicates and celebrates the participation of undergraduate students from across the University in scholarly inquiry, research and creative endeavors. Students from all Penn State campuses are eligible to enter one of three formats for sharing research and creative inquiry in the Undergraduate Exhibition in public sessions.
Pugh Lab Undergraduate Researcher Olivia Muly presented her poster Wednesday evening at this Exhibition. The poster outlined her Pugh Lab research on characterizing DNA regulatory elements in yeast under high sugar and oxidative stress conditions. Every cell regularly reads its genetic code to produce mRNA in a process called transcription, with the aim of ultimately producing thousands of proteins. Eukaryotic organisms use a system of activator and repressor proteins to modulate transcription. The goal of her research is to understand how genes are regulated by exposure to environmental stress and subsequent characterization of protein/DNA interactions genome-wide through ChIP-exo technology.
We are very proud of Olivia and are excited to continue fostering her research. Congratulations on your first poster presentation Olivia!
For more information about our student researchers or opportunities to join the lab please click here!
Pugh lab has a job opening for a Research Technician!
This is a full time staff-level position with Penn State and thus includes all the benefits and pay scales pertaining to university employees. The start date would be early May 2018. Our research techs conduct molecular biology experiments such as; ChIP-seq, PCR, gel electrophoresis and cell culturing using standard protocols that we train them in. Competitive applicants have a GPA >3.0, have excelled in molecular biology laboratory classes, and have a commitment time frame of 1-2 years. Past technicians utilized this opportunity to gain additional laboratory experience before heading off into other professional training programs.
Applications must be submitted electronically and include a cover letter and resume. To submit an application click here. Recent university graduates are welcome to apply!
Please contact our Lab Manager Kylie Bocklund should you have any questions. email@example.com